BIO 2133 Lecture Notes - Lecture 1: Chromosome, 40 Minutes, Microscope Slide
5 Feb 2016
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Document Summary
The purpose of this lab is to understand the structure of polytene chromosomes, and compare their structures under different conditions. Polytene chromosomes are produced by endomitosis, which is the repeated duplication of homologous pairs of chromosomes without chromosome separation or nuclear division. As a result, they grow to an extremely large size and have many strands. The chromosomes will be examined as is, and after the application of a heat shock treatment. The purpose of the heat shock treatment is to induce a phenomenon known as chromosomal puffing, which results in enlarged areas of active gene transcription. The polytene chromosomes, with and without heat shock treatment, will be studied thoroughly and presented in this report. In both sets of polytene chromosomes, that being those experiencing and not experiencing heat shock treatment, the general outline of the procedure was the same.
