BIO 1140 Lecture Notes - Lecture 9: Calcium Atpase, Intermembrane Space, Cellular Respiration

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BIO 1140
Lecture 9: Mitochondria Part 1
*add chloroplasts to learning objectives
Energy Flow in an Ecosystem
Is more than just the powerhouse of the cell
Metabolism
Response to stress: increase in temp, pH, etc
Cell death: making sure that a cell can die when it has to and that it happens in a
structured manner
Calcium storage
Studying structure and function
Double membrane
Intermembrane space
All parts of mitochondria have different functions
Build enough potential energy so that the protein that is essentially a motor that needs
to be fueled to accomplish a rotation and when it does it is able to phosphorylate ADP
into ATP
Is an integral and a primary active transport channel protein and has a quaternary
structure and is an enzyme (ATP synthase), can also work in reverse (ATPase to
hydrolyse ATP to produce ADP)
Potential energy in ATP is mostly stored in the bonds between the phosphate groups
Enzymes
Are protein with a few exceptions that are partially or completely RNA
Have a binding area that allows them to bind with their substrate to then catalyse the
reaction
Do’t eed to kow all of the differet lasses of ezyes
Metabolic Function of Mitochondria
Glycolysis: important first step of cellular respiration that occurs in the cytosol
ATPase is powered by H+ gradient
Do’t sped tie learig differet reatios that take plae, fous o how the
concentration is built
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Document Summary

Metabolic function of mitochondria: glycolysis: important first step of cellular respiration that occurs in the cytosol, atpase is powered by h+ gradient, do(cid:374)"t spe(cid:374)d ti(cid:373)e lear(cid:374)i(cid:374)g differe(cid:374)t rea(cid:272)tio(cid:374)s that take pla(cid:272)e, fo(cid:272)us o(cid:374) how the concentration is built. Investment and phase and a payoff phase: use atp in investment phase, gain atp and other molecules in payoff phase. Krebs cycle: only 1 atp during citric cycle but 3 adh and 3 h+ and 1 fadh2. In both mitochondria and chloroplasts: use electron carriers and build an electron gradient. Proton motive force: the stronger your gradient, the abler you are to phosphorylate adp into. Rate limiting steps: steady input of oxygen otherwise the gradient is even and there is no drive to get the gradient across and the force is lost, lack of adp which is transported there and is recycled after use. Mitochondria needs a lot of calcium and is an excellent calcium storage mechanism.

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