BIO 1140 Lecture Notes - Lecture 3: Confocal Microscopy, Scanning Electron Microscope, Transmission Electron Microscopy

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Smallest unit with the capacity to live and reproduce. Cell theory mid 1800s all organisms are composed of one more cells the cell is the basic structural and functional unit of all living organisms (schwann) cells arise only from division of pre-existing cells (virchow) Diversity: wide range of sizes, simple or complex shapes related to function, general or very specialized role. Something that limits from the inside to the outside: basic chemistry, metabolism (atp, dna. Relevant units: 1 micrometre = 10^-6m, 1 nanometre = 10^-9 typical cell sizes, prokaryote = 1-5 micrometres, eukaryotes = 10-100 micrometres. Why are cells small: if a cell is large, it is susceptible to damage, surface area to volume ratio. Dark field microscopy: light illuminates the specimen at an angle, and only light scattered by the specimen reaches the viewing lens of the microscope, this gives a bright image of the ell against a black background.

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