BIO 1140 Lecture Notes - Lecture 3: Phagocytosis, Turgor Pressure, Nuclear Lamina
8 Feb 2018
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Document Summary
Biologists use microscopes and the tools of biochemistry to study cells. Robert hooke: begin to look at cell walls. Specimen preparation in microscopy can introduce artifacts, structural features see in micrographs that do not exist in the living cell. Confocal and deconvolution in microscopy have produce sharper images of 3d tissues and cells. Microscopes first used by renaissance scientists and most used today are light microscope (lm) Most important tools of cytology- study of cell structure. Ration of object"s image size to its real size. Lm can magnify effectively to about 1000 time the actual size of the specimen. At greater magnification additional details cannot be seen clearly. Minimum distance two points can be separated and still be distinguished as separate points. Lm cannot resolve detail finer than about 200 nanometers (nm) regardless of the magnification. Difference in brightness between the light and dark areas of an image. Enhance by using: staining or labelling cell components.
