BIO 1140 Lecture Notes - Lecture 5: Reducing Equivalent, Multiprotein Complex, Atp Synthase
3 Nov 2017
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Topic 5: mitochondria and energetics 2/13/2015 10:22:00 pm. Explain what is the role of mitochondria in a cell with regards to energy production. Understand the importance of calcium: availability, storage, cellular functions. Understand key concepts of cellular energetics in relation to mitochondria. Essential for many physiological functions: metabolism, response to stress, cell death, calcium storage. Fe/s complex assembly (complex i and ii; metalloproteins, ferredoxins, etc. ) Methods to isolate mitochondria: centrifugation, subcellular fractionation, differential centrifugation, density gradient centrifugation, equilibrium density centrifugation. The objective: power up the f type atpase the f1f0 atpase: cristae increase surface area and gives more room for f type atpases to create a bigger atp gradient larger atp synthesis capacity. Glycolysis: occurs in the cytosol outside the mitochondria: transforms glucose into nadh and 2 pyruvate molecules, makes 2 atp, depends on the cell"s metabolic requirements and on the availability of oxygen.
