BIO 1140 Lecture Notes - Lecture 20: Proofreading, Dna Repair, Dna Replication
8 Oct 2016
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Both strands of dna are separated, replication occurs simultaneously on both parental strands. Helicase protein that wraps around 2 parental strand, it is at the fork. Origin of replication is at the center of the fork. Single stranded binding protein attach themselves to each parental strand once they"ve been separated to make sure the dna doesn"t recoil. Topoisomerase downstream and upstream of replication forks, cuts the strand a little to relieve pressure so it doesn"t supercoil. Goes to origin of replication, adds short sequence of rna nucleotides (6-10), short segment now appears double strand in order to recruit dna poly iii. Primase adds primer 5"-3", on one strand we need to only add one, on the other strand need to add several primers. Replication always occurs from origin towards the fork (5"-3") If you have a dna strand with the following sequence, what would be the sequence of.
