BCH 2333 Lecture Notes - Lecture 10: Alpha Helix, Molecular Phylogenetics, Ribonuclease
4 Mar 2016
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Delta g = -50kj/mol is very small o. About the same order of magnitude as delta g and so they are still essential to protein folding. Increase temp = decrease strength of h bonds. Protein folding occurs on a complex energy surface. Folded states are not always lowest e states they can adopt o o. Proteins can sometimes end up in aggregation o o o. Catalysts and chaperones can bring a protein from one folded state to another. Page, native and denaturing *** sds page http://www. youtube. com/watch?v=3crzy7jb9fq. Trypsin breaks peptide bonds; it reacts at carbonyls of lys (k) and arg (r). Trypsin will not digest the protein if pro is next to the aa. K & r occur frequently so proteins are cleaved into small fragments. Edman degradation classical method o o https://www. youtube. com/watch?v=7nubm99yoyw. Successive rounds of n-terminal modification, cleavage, and identification. o o. Can be used to identify protein with known sequence.
