HIS108 Lecture Notes - Lecture 2: Haemophilus Influenzae, Kary Mullis, Base Pair
Document Summary
Biology 1a03 theme 4 module 3 and 4. Replication in a tube (in vitro: pcr developed by kary mullis in 1985, dna primers 15-30 nucleotides in length, deoxyribonucleotides (dntps, heat-tolerant dna polymerase - taq polymerase (isolated from the bacterial species. Thermus aquaticus which are adapted to live in hot springs with temperatures as high as. 1995: first multicellular organism genome of c. elegans being sequenced in 1998, then. Drosophila in 1999, and the whole human genome in 2000. Phase 1: random sequencing of the dna in each fragment. It is believed that this discrepancy is due to the delicate nature of the rna backbone of. Rna viruses and retroviruses (being more prone to damage and breakage), in addition to the fact that there is no proofreading capability in rna genomes. Somatic versus germline mutations: genetic information can be mutated in either somatic or germline cells.
