BIOC 302 Lecture Notes - Lecture 12: Plasmid, Primase, Dna Supercoil
Document Summary
G goes with c, a goes with t anything else doesn"t it in the acive site. The enzyme sill makes mistakes though, it"s not perfect. Ligases only like dna-dna substrates, it cant work at the beginning when there is rna/dna substrates. Dna ligase: this is to seal the dna at the end of nick translaion put amp on a 5" phosphate amp is a good leaving group. Can only process a short strip of template at a ime. This is why you need dna polymerase iii. Has high processivity processes ~3000 at a ime (or something like that) A current model of replicaion of dna pol iii implicates dual funcion: one part is involved in leading strand synthesis, the other is involved in lagging-strand synthesis. Replisome all the stuf necessary for replicaion. Dna polymerase: disconinuous on botom, coninuous on botom. Helicase: to separate the strand: helicase needs atp because dna wants to be double stranded.
