BIOC 302 Lecture Notes - Lecture 22: Intron, Stem-Loop, Rna Polymerase Ii

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sapphirehyena95

11 Apr 2016

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Eukaryoic is compartmentalized they have a nucleus which separates the process of translaion and transcripion. Make mrna in the nucleus and transport it out to translate it in the cytoplasm. People typicaaly report a gene sequence as the dna sequence that would be the mrna if the t"s were writen as u"s (the coding strand) The template strand is complementary and aniparallel to the coding strand. The template (3" to 5") is used to make the produce (5" to 3") In eukaryotes, introns would also be present in the dna they need to be spliced out to give mature dna. Rna polymerase (rnap) separates the strands of dna and polymerases rna in a 5" to 3" direcion. Bacterial rna polymerase is simple, with 5 subunits ( 2, , ", ) Eukaryotes have three rna polymerases that are much more complex.

Related Questions

During transcription in eukaryotes, a type of RNA polymerase called RNA polymerase II moves along the template strand of the DNA in the 3'â5' direction. However, for any given gene, either strand of the double-stranded DNA may function as the template strand. Which of the following initially determines which DNA strand is the template strand, and therefore in which direction RNA polymerase II moves along the DNA?

A. the location of specific proteins (transcription factors) that bind to the DNA

B. the specific sequence of bases along the DNA strands

C. the location along the chromosome where the double-stranded DNA unwinds

D. the position of the geneâs promoter on the chromosome

E. which of the two strands of DNA carries the RNA primer

1. A region of a chromosome contains two strands of DNA, yet only one is used to transcribe a gene because

A. all genes are found on the same strand of DNA in a chromosome.

B.both strands are transcribed, but only one strand of DNA contains a reading frame.

C.RNA polymerase binds to the terminator, stopping transcription on one strand of DNA.

D.RNA polymerase binds to the promoter, which precedes the gene only on the template strand of DNA.

E.the start of transcription is random along the chromosome and doesn't always occur on a gene.

2. In eukaryotes an mRNA molecule can only be translated by one ribosome at a time.

True or False

3.The process by which cells use the information of RNA molecules to make proteins is transcription.

True or False

What are the three processes in post-transcriptional processes that must be completed before transcripts can be translated in eukaryotes?

A.	Binding of snRNPs, addition of a poly A tail, splicing of introns
B.	Binding of snRNPs, transporting, synthesizing of ribose
C.	Capping, transporting, synthesizing of ribose
D.	Binding of snRNPs, capping, splicing
E.	Capping, splicing, addition of a poly A tail

Which of the following key differences between prokaryotic and eukaryotic transcription are TRUE? Check all that apply.

	A. prokaryotic transcription occurs in the cytoplasm, while eukaryotic transcription occurs in the nucleus
	B. eukaryotic transcription is carried out by 1 RNA polymerase, while prokaryotic transcription is carried out by 3 different RNA polymerases
	C. eukaryotic transcripts are processed, while prokaryotic transcripts are translated while transcription is still occurring
	D. prokaryotes have sigma factors for specificity of binding whereas eukaryotes have multiple general transcription factors
	E. prokaryotes have to worry about unpackaging the DNA for transcription, while eukaryotes have almost naked DNA not bound up by nucleosome

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BIOC 302 Lecture Notes - Lecture 22: Intron, Stem-Loop, Rna Polymerase Ii

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