KIN406 Lecture Notes - Lecture 8: Membrane Potential, Cardiovascular Disease, Cytosol
13 Mar 2017
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Document Summary
Ubiquitin-proteasome system (ups: mainly responsible for basal turnover of short lived, damaged, and misfolded proteins, recognizes targeted proteins by a poly-ubiquitin (ub) chains, the ubiquitin process involves 3 main enzymes, e1- activation, e2 - conjugation, e3 - ligation. Activation, conjugation, litigation: the proteasome does all the degrading (final step). But we have to get there first: protein chaperones (hsp70) this protein and says it is mis-folded, finally ub is added to the protein as a chain, making it targeted to be degraded. Ubiquitin-proteasome system and pathophysiology: pathological states can arise if the ups does not function properly. Accelerated removal of specific proteins in cancer: western blot: protein expression. The darker the band the higher expression: low amount of p53 (tumour suppressor gene, treat them with proteasome inhibitors mg132 and lc. This makes p53 higher: shutting down degradation and the proteasome.
