MBIO 4440 Lecture Notes - Lecture 13: Massive Parallel Sequencing, Sanger Sequencing, Pyrosequencing

• School: University of Manitoba
• Department: Microbiology
• Course: MBIO 4440
• Professor: Silvia Cardona

Document Summary

So far, we have learned that the method of emulsion pcr allows the synthesis of sequencing features immobilized on a bead. The details of bridge pcr are not as available as emulsion pcr because. Bridge pcr is a proprietary method developed by illumina. However, the principle and the goal is the same: to produce millions of identical ssdna molecules immobilized on a surface that can be later sequenced. To understand bridge-pcr we will watch a movie developed by illumina as part of their marketing strategy. Finally, we can discuss sequencing reactions and detection methods used by ngs platforms. The table shows most ngs platform use the concept of sequencing by synthesis, that is the signal comes from the synthesis of ssdna complementary to template strand. As you know, for sanger sequencing, the synthetized ssdna molecules are irreversibly terminated at different lengths by the use of dideoxynucleotides.