BIOL 205 Lecture Notes - Lecture 13: Sulfate Adenylyltransferase, Rna Polymerase Ii, Apyrase
Document Summary
Next generation sequencing methods- pyrosequencing: add a single dna to a single bead, place bead in one of. Dna synthesis: uses luminescence to detect sequence. Principle of pyrosequencing pyrosequencing technology, which is based on the principle of sequencing by synthesis. A sequencing primer is hybridized to a single-stranded pcr amplicon that serves as a template, and incubated with the enzymes, dna polymerase, atp sulfurylase, luciferase, and apyrase as well as the substrates, adenosine 5" phosphosulfate (aps), and luciferin. The first deoxribonucleotide triphosphate (dntp) is added to the reaction. Dna polymerase catalyzes the incorporation of the deoxyribo-nucleotide triphosphate into the. Dna strand, if it is complementary to the base in the template strand. Each incorporation event is accompanied by release of pyrophosphate (ppi) in a quantity equimolar to the amount of incorporated nucleotide. Atp sulfurylase converts ppi to atp in the presence of adenosine 5" phosphosulfate (aps).
