MICR 2420 Lecture Notes - Lecture 19: Antibiotics, Antitoxin, Housefly

• School: University of Guelph
• Department: Microbiology
• Course: MICR 2420
• Professor: Ajila Chandran Matheyambath

Document Summary

Cholera symptoms: begin (cid:494)out of the blue(cid:495) with sudden onset of explosive, watery diarrhea, painful abdominal cramping soon starts, diarrhea continues, without fluid/electrolyte replacement, death soon occurs, with treatment, disease is self-limiting. Adp ribosylating toxins: diphtheria toxin, ribosylates elongation factor 2, blacks ribosome function; cell dies, cholera toxin, ribosylates to overactivate adenylate cyclase, camp activates ion transport; water follows. Host cell co-operation: excreted toxin binds to gm1 gangliosides on host cells, following binding the whole toxin is take up into an endosome, the ct travels via retrograde transport first to the golgi and then to the er. In the er, the toxin is processed leading to the release of the a1 subunit into the cytosol. Diagnosis: clinical symptoms, measurement of serum antibodies using elisa. Transmission: bacteria transmitted via contaminated water and food, carriers: houseflies and other insects, person to person transmission. Immunological: local mucosal immune response to v. cholerae, serological antivibrio antibodies, antitoxin antibodies, to ease symptoms, oral rehydration, iv.