MCB 2050 Lecture Notes - Lecture 5: Huntingtin, Taq Polymerase, Bioinformatics
26 Jan 2017
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Techniques for dna/rna analysis: gel electrophoresis, dna restriction mapping, southern blot (dna analysis, northern blot (rna analysis, polymerase chain reaction (pcr, dna sequencing. Gel electrophoresis: uses an electric eld to separate macromolecules in agarose (dna/rna) or polyacrylamide (dna or protein) gels. Separation of a mixture of dna (orrna) fragments in porous gel matrix (agarose or polyacrylamide) in an electric eld. Since dna is negatively charged, it will migrate toward the anode (positive electrode) Their rates of migration are inverse proportional to their sizes (i. e. length the shorter ones move faster than the longer ones. Applications: determine sizes of dna fragments, isolate dna fragments for cloning, in preparation for southern/ Restriction mapping: (for physical mapping of dna based on relative location of. Mapping of restriction sites on dna: an example of physical mapping . Applications: relative positions of restriction site son a dna used as markers. provides overview of organization of a dna fragment.
