BIOL 1090 Lecture Notes - Lecture 22: Pyrophosphate, Unified Atomic Mass Unit, Transfer Rna

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Storage, replication & repair of genetic material. Binds ribosomes and is continuous and is continuous with rough er (rer) Contains integral membrane proteins that connect to nuclear lamina. Allows limited movement of molecules between nucleus and cytoplasm. Bound to inner surface of nuclear envelope (ne) Via prenyl goru[ linked to lamin polypeptide. Mutations in a lamin gene (lmna) implicated in hutchinson-gilford progeria syndrome, a rare disease that cause premature aging in children. Inner & outer membranes of ne fuses at pores. Fits into the pore; reduces functional diameter to approx. Passive diffusion of small molecules (<9nm diameter) across npc. Regulates movement of larger molecules import of nuclear proteins and rnas (rrna, trna) into nucleus exports of rnas (mrnas, trna), ribosomal subunits, proteins from nucleus. 40,000 dalton size: e. g. gfp (27kd) are able to diffuse freely in & out. Cargo protein contains a nuclear localization signals (nls) Stretch of positively charged amino acids near c-terminus of protein.

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