BIOC 2580 Lecture Notes - Lecture 3: Silica Gel, Lysine, Protein Structure
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In order to be completely deprotonated the pka needs to be one below. Answer: histidine since it has the same pka value. Calculate the charge of the side chain of lys at ph 9. 8. Use henderson hasselbach equation: ph = pka + log[dep]/[pro] Amino acid analysis helps to determine protein structure. Can be qualitative (tells you what is present) Can be quantitative (tells you how much is present) Can be preparative (separated components can be recovered for further experiments. Partition chromatography is an important method for separating components of a mixture. Particles of solid are chosen with a specific property, eg. silica gel has ho-si-oh groups that can hydrogen-bond to polar amino acids. Liquid solvent or buffer flows pas the particles and is non-polar. Polar amino acids p spend more of their time hydrogen bonded to silica and move slowly. Non-polar amino acids n spend more time in solvent, and move almost as fast as solvent.