BIOL207 Lecture 22: b207lect22_1.pdf

• School: University of Alberta
• Department: Biology (Biological Sciences)
• Course: BIOL207
• Professor: Cameron Harrington

Document Summary

Lecture 22 - polymerase chain reaction (pcr: background, how to do pcr, some uses of pcr, background, dna replication, dna and temperature dsdna denatures when heated. During renaturation any complementary pieces of ssdna can pair: enzymes and temperature. Enzymes from e. coli work best at 37 c. Enzymes from thermus aquaticus work best at 72 c http://tolweb. org/ Synthetically produced pieces of ssdna usually about 20 nt long. And serve as primers for in vitro dna replication: how to do pcr, timeline. Kary mullis wins nobel prize www. nobelprize. org/nobel_prizes/ chemistry/laureates/1993: principle in vitro exponential amplification of a small region of dna, limiting factors. You must know the sequence at both ends of the region to be amplified. The region to be amplified can"t be > 5 kb: the reaction. After ~30 cycles the tube will contain: original template dna some unused dntps and primers some variable length dna lots of constant length dna.