BIOL207 Lecture Notes - Lecture 28: Agarose Gel Electrophoresis, Ethylenediaminetetraacetic Acid, Thymidine Triphosphate
21 Apr 2018
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Detergents to dissolve lipid membranes and denature proteins. Na+ helps stabilise the negatively charged dna molecule. Supernatant is mixed with ethanol which causes dna to precipitate. Small pellet of dna collected through centrifugation. Dna is dissolved in water (with edta and ph buffer) Isolating or detecting a specific sequence by pcr. Pcr contains 20 different primers to initiate replication. Experimenter can therefore control exactly what region of the dna template to amplify by specifying the sequence of primer. Nucleotides (datp, dgtp, dctp, dttp) a dna template. Successful pcr reactions have been conducted using only 1 dna template however in practice most successful pcr reactions have thousands of templates. Sometimes whole cells can be put directly in the pcr reaction. Thermal cycling: exposure of reaction to a series of precisely defined temperatures. First heated to 95 degrees- hydrogen bonds between dna strands break. Two single stranded dna molecules are produced from each double helix.
