BIOL207 Lecture Notes - Lecture 8: Model Organism, Reciprocal Cross, Telomerase Rna Component
29 Sep 2013
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Lecture 8: usefulness of gene mutations | lecture 9: mendel invents. Induce a lot of mutations and isolate individuals with desired mutation: problem: screening in multicellular organisms is a lot more difficult than screening unicellular organisms. Screening auxotrophs of neurospora: steps for screening of drosophila: 1 expose male flies to mutagen through ems in food or x-ray. Somatic mutations are interesting, but not what we want for the purpose of screening because the mutation dies with the individual we"re interested in the germ line mutations here. So only one mutation is passed onto the offspring. Individuals with desired phenotype are collect and made into a permanent stock while the others are discarded. Targeted gene knockout: replace wt allele with mutant one, example: mice. Dna started losing telomeres and fusion of chromosomes with telomeres only on one side occurred (to make it so that there are telomeres on both ends: protein"s way of fixing this mutation.
