L & S C30Y Lecture Notes - Lecture 11: Centenarian, Insulin-Like Growth Factor 1 Receptor, Model Organism
27 Feb 2017
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Probably the second most important genetic experiment after. The beadle-tatum experiment: can one isolate mutants in biochemical processes, specifically, a mutant that will not grow without a specific amino acid, step 1: mutagenesis. Yeast can live as haploid or diploid cells. They exist in two sexes, which yeast biologists do not call sexes, and instead call mating types . Two haploid yeast cells of different mating type can fuse, and form a diploid. That diploid can do one of two things: grow mitotically, as a diploid, go through meiosis, and form 4 haploid cells, each of which can go back to living as haploid cells. Capable of synthesizing everything necessary from simple salts. Antonym: auxotrophic needing a specific nutrient for growth. Humans are auxotrophic for lysine, we cannot live without it in the diet. E. coli is prototrophic for lysine, it can make it just fine. It will grow in inorganic salts and one vitamin.
