BIOCH200 Lecture Notes - Lecture 28: Net Energy Gain, Concerted Reaction, Glyceraldehyde 3-Phosphate Dehydrogenase

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Biochem 200 - lecture #28 - reactions of glycolysis. One molecule of glucose produces two molecules of gap: would need to produce at least 3 atp to get a net gain, every reaction described from gap to pyruvate happens twice per glucose. Stage 2: glyceraldehyde-3-phosphate to pyruvate: 4 atp are generated for every glucose, net profit of 2 atp. Generating a high-energy product! (nadh: the golden reaction of glycolysis. Large - g of hydrolysis attached to carboxylates: 1,3-bpg is a high-energy intermediate because it is an acyl phosphate (phosphates. Used to make atp, a high-energy molecule: thermodynamics/equilibrium, you get 2 high energy molecules out of this reaction, not a phosphate transfer (an addition) Reaction i: a (cid:1372) b g " > 0. Reaction ii: b (cid:1372) c g " < 0. 1st reaction is made more favourable by the 2nd reaction (2nd reaction pulls the: indirect coupling. Need to have the reactions happen in a group (concerted reaction)

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