MBB 222 Lecture Notes - Lecture 16: Reaction Rate, Enzyme Kinetics, Reaction Rate Constant

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A general, quantitative way of looking at any enzyme"s behavior. Enzyme kinetics: the substrate concentration affects the rate of a reaction. The reaction rate or velocity (v) is a function of the change of substrate concentration over time. At equilibrium, the reactants are being converted to products at the same rate as the reverse reaction and there is no net change in the concentration of s and p. Follow v by measuring the increase in product concentration over time for different initial substrate concentrations, [s]n. The amount of product formed at different initial substrate concentrations ([s]n) is plotted as a function of time. Enzyme concentration is kept constant and is usually far below. The initial velocity (v0) for each substrate concentration is determined from the slope of the curve at the beginning of a reaction, when the reverse reaction is insignificant. Now plot reaction velocity as a function of initial substrate concentration.

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