BLG 311 Lecture Notes - Lecture 9: Emission Spectrum, Post-Translational Modification, Photobleaching
9 Dec 2016
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Document Summary
After a few light microscopes b/c available in the 1838, schleiden and schwann proposed that all plant and animal tissues were aggregated of individual cells cell. Limit to the resolution of a light microscope is set by the wavelength of visible light. The light microscope can resolve details 0. 2 apart: 0. 4 (for violet) to 0. 7 (for deep red) Smallest object we can see in a light microscope: bacteria and mitochondria. Limitations: light waves travel thru optical system, so when they interfere each other it causes optical diffraction effects. If two trains of waves reach the same point by diff paths in phase. With crests and troughs matching, reinforce each other and increase brightness. If two trains of waves cancel each other out out of phase. If a small object, below the resolution limit, itself emits light, then we may still be able to see or detect it: we can see a fluorescently labeled microtubule.
