BCHM 102 Lecture Notes - Lecture 32: Antigen, Restriction Fragment Length Polymorphism, Dominance (Genetics)
2 Dec 2016
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Lecture thirty-two - biotechnology and human disease ii. Lecture objectives: understand polymerase chain reaction (pcr) and its application to biotechnology. Introduce methods of studying gene expression at both the mrna and protein level: pcr (?, microarrays, elisas, western blots, proteomics, understand gene therapy and the production of transgenic animals as model organisms. Polymerase chain reaction (pcr: only a small amount of dna is necessary in pcr compared to rflp. Pcr is an in vitro (test tube) method of amplifying a dna sequence using dna polymerase: no host cell required. Synthesizes millions-billions of copies of a specific nucleotide sequence in a short period of time: 2-3 hours, no cloning required. Each cycle of pcr doubles the amount of dna in the sample: exponential (2n) increase in amount of target dna in short period of time. Pcr requires primers that bind to dna on either side of sequence of interest. Primers are short oligonucleotides (20 35 nt long)
