BIOL 4241 Lecture Notes - Lecture 7: Polyclonal Antibodies, Passive Immunity, Ouchterlony Double Immunodiffusion
27 Mar 2017
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Research question: to see if its possible to use immunological differences of proteins to determine the evolutionary divergence of hominoidea genus. Specimens that were used to collect samples of albumin in their experiment were old world monkeys and the 6 living genera of apes known as hominoidea. Steps of wilso(cid:374) & sari(cid:272)h"s method: collected albumin samples from all species that were just mentioned, made antiserum, tested for purity using immunodiffusion and immunoelectrophoresis, used microcomplement fixation to determine antibody-antigen reaction. Serum samples collected from all the living genera of apes and from six representative genera of. Old world monkeys: albumin was purified from individual chimpanzee, gibbon and human serums by method of hoch and chanutin. It was raised to ph 8. 0 to 8. 3 by addition of naoh, and the solutions were examined by electrophoresis in 0. 6 per cent nacl. Serum albumin: a type of globular protein found in vertebrate blood, most abundant protein in mammals.
