BIOC 212 Lecture Notes - Atp Hydrolysis, Garland Science, Phosphocreatine

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taupehare180

22 Aug 2012

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Glycolysis: storage, export, conversion into other metabolites. Creatine phosphocreatine: an easy means of getting energy through atp hydrolysis, reaction is carried out by creatine kinases, when it nears equilibrium in the cell. Consumed rapidly when low in atp & recharged at rest: enough are stored for 2-10 seconds of effort (sprint, anaerobic reaction. Chloroplasts: undergoes photosynthesis, storage of starch polymers. Lipid droplet = very hydrophobic: 3 fa chains, 1 glycerol, linkage = ester bond. Fat = good means of energy storage: conversion of glucose into fat then this fat can be stored into droplets, when you don"t eat & system needs energy, you use these chains to access your energy reserve. Energy storage: glycogen (animals) & starch (plants) Stocks are limited (1 day in human) Figure 2-78 molecular biology of the cell ( garland science 2008)o fat (mainly triacylglycerols) Excellent stocks of it! (~ 1 month in human)

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Creatine (Cr) is made in the liver and kidneys and then transported to the muscles, where it serves as a high energy storage compound via phosphorylation by creatine kinase. Two forms of this enzyme exist, mitochondrial and cytoplasmic. In mitochondria there is net synthesis of creatine phosphate (CrP). In the cytosol, the phosphate is transferred from CrP to ADP to supply ATP to power the muscles. Table 3.2 of your G&G text lists ÎGËÌ for hydrolysis of creatine phosphate: -43.3 kJ/mol at 25Â°C. creatine phosphate + H2O ----> creatine + Pi

a. Calculate the KeqÍ for this hydrolysis.

c. In mouse muscle cells at 25Â°C, [Cr] is 10 mM, and [CrP] is 20 mM. What concentration of Pi would be needed to maintain this level of phosphorylation by reversal of the hydrolysis reaction? Is this concentration of Pi a reasonable expectation? Why?

d. Coupling ATP hydrolysis (ÎGËÍ-35.7 kJ/mol at 25Â°C) to an unfavorable reaction is often used to drive biochemical reactions. Write the chemical equations for the CrP reverse hydrolysis and ATP hydrolysis and add them together to give the coupled reaction. Then calculate ÎGËÍfor the coupled reaction. Is this reaction favorable? Justify your answer.

e. Considering ÎGÍin the muscle cells rather than under 1M standard state conditions, what ratio of ATP/ADP would be needed inside mitochondria to make the coupled reaction for CrP synthesis produce the concentrations of Cr and CrP observed in mouse muscle? Is this ratio reasonable? Why?

crimsonduck531

Use the free-energy values given below to predict whether the conversionof creatine phosphate to creatine will provide enough energy to phosphorylate ADP. Determine the net reaction and then calculate the overall ÎG⁰'. Is net reaction spontaneous?

Creatine Phosphate + H20 -> Creatine + Pi

ÎG⁰'= -43.1 kJ/mol

ADP + Pi -> ATP + H2O

ÎG⁰'= 30.5 kJ/mol

BIOC 212 Lecture Notes - Atp Hydrolysis, Garland Science, Phosphocreatine

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