BIOL 202 Lecture Notes - Lecture 9: In Situ Hybridization, Expression Vector, Gamete
31 May 2016
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Lecture 9a bioinformatics and genomics - may 17, 2016. The quiz today and tomorrow covers lectures 7 10 of hipfner only. Bioinformatics & genome annotation: predicting genes and analyzing the genome. Bionformatics and comparative genomics: within a genome duplications and gene families, between organisms synteny and core conserved genes. Functional genomics: transcriptomics ests, microarrays, rnaseq, chromatin immunoprecipitation (chip) Massively parallel sequencing: can sequence >1,000,000 dna molecules in one experiment. There are two things that helped revolutionize our ability to sequence dna: robots = much more efficient, in one set of reaction, sequencing a million pieces of dna. This enabled scientists to do shotgun sequencing: random sequecning millions of random pieces of genomic dna. So have genome cut many genome copies into random fragments sequence each fragment overlap sequence reads to make a continuous longer sequence called a contig overlap contigs for complete sequence. In 2001, they released the entire sequence of the human genome.
