BIOL 202 Lecture Notes - Lecture 27: Tata-Binding Protein, Galactose, Chromatin
5 Nov 2015
School
Department
Course
Professor
Document Summary
Testing for the presence of regulatory sequences in dna: reporter gene assays: take the promoter of interest, place it before a reporter gene. March 16th, 2015: a reporter gene is a protein whose levels can be easily detected - whose amount is easily measured, e. g. gfp by fluorescence, beta-gal by x-gal (blue substrate) Cell/tissue-specific transcriptional regulation: efficient transcription requires binding of specific transcription factors to enhancer sequences. Recruit cofactors (proteins that don"t bind dna directly) which help. Bending and looping of dna by architectural proteins means they"re not actually physically far away general tfs/rna pol ii to be recruited to promoters (cofactor), which in turn assists in bringing rna pol ii to the promoter, initiating transcription. Enhanceosome of beta interferon: regulatory proteins recruit cbp o. Binding of insulator proteins prevent enhancers from acting. Enhancer-blocking insulators inappropriately on promoters beyond the insulator.
