BIOL 201 Lecture Notes - Lecture 16: Mitosis, Cell Cortex, Paclitaxel

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19 Apr 2018
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Dynamic instability can be directly observed in vitro. Growth rate can be calculated from the position/length changes of the tubulin as a function of time. When cells want to structure their cytoskeleton in some particular way, they have to manage & control the dynamic instability of the microtubules. Growth rate needs to be controlled & so does the point of collapse. Polymerases --> proteins that make microtubules grow faster. +tip proteins --> recognize the tip/cap of microtubules and allow cells to target other proteins to the tips. Severing enzymes --> proteins that can cut microtubules in half. Nucleation factors --> proteins responsible for creating new microtubules. Experiments that showed this took cells and shifted them to 4 degrees. Cold temperature causes all of the microtubules to collapse, so when shifting the cell temperature all the microtubules go away. When rapidly heating the cells back up, you see a rapid blooming of microtubules from the centrosome.

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