BIOL 115 Lecture Notes - Lecture 19: Transgene, Rhodopsin, Doxycycline
Document Summary
I. immediate early genes: transcribed then translated immediately when an action potential arrives at the post-synaptic cell. A. to hold the memory of something like foot-shock, the particular cells need to be activated without using other cells. This is done with c-fos, a protein called chr2, and an antibiotic called doxycycline/dox: step one: c-fos is a transcription factor, it can regulate the transcription of other genes. Chr2-eyfp **know this: enhanced yellow uorescent protein, a protein modi ed from green uorescent protein that is found in jelly sh, glows when hit at a particular wavelength of light. In summary: an experience activates a certain population of cells in the hippocampus, these cells express c-fos which activates the c-fos promoter and leads to tta expression. 4. tta binds to tre and leads to chr2-eyfp expression the cells coded for this experience glow in green/yellow under the microscope because of.
