NEUR 3200 Lecture Notes - Lecture 4: Intron, Exon, Amplifier
8 Mar 2016
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Membrane potentials are established by ion transporters. Sodium and potassium channels were ion specific, voltage gated and had large pores. Small glass pipette full of saline touched to membrane, slight suction creates seal, preventing ion flow. Electrical current measured with an ultrasensitive electronic amplifier connected to the pipette (cell attached recording) Strong suction will create a whole in membrane. Measures electrical potential of whole of inside cell and allows introduction of drugs into cell (whole cell recording) Mild suction followed by retraction removes a vesicle of membrane from potential and the exposure of the vesicle to air opens the vesicle. Can introduce exposed surface into a new medium completely changing the intracellular environment (inside out recording) Both are voltage gated (must have molecular voltage sensors) Both are ion selective (pore must discriminate between ions. Both are uniquely sensitive to drugs (must have binding sites for tredotoxin and tetraethyl ammonium. Only sodium channels inactivates (must have molecular inactivation mechanism)
