Physiology 3140A Lecture Notes - Lecture 11: Extracellular Fluid, Inside Out Music, Sodium Channel
28 Dec 2017
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Glass pipette: dip it into recording chamber containing neurons or cells to be studied. Recording electrode filled with solution, connected to wire electrically. When pipette is approaching a cell, you can monitor the resistance of the tip: as you get closer to the cell, the resistance increases, the membrane is insulating, allows no passage of ions. After forming the giga seal, perform a strong suction. Now the pipette solution and the cell"s intracellular medium is continuous: the cell is so small compared to the solution in the pipette, the cell content is dialyzed by the pipette solution. Record voltage in mv (record membrane potential changes) Close to native state of the cell! (if you do not inject current, it is how the neuron will behave) I= gv (ohms law current proportional to the conductance; voltage is controlled here) Study the current under a fixed voltage, called voltage-clamp (v-clamp) recordings.
