BIOL 2P02 Lecture Notes - Lecture 22: Dna Mismatch Repair, Nobel Prize, Dna Replication
14 Dec 2016
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Total lesions from all mechanisms in humans depend on tissue and the environment: approximately 105 to 106 lesions/cell/day, usually non-consequential takes a while to kill you. Know: common dna damage and lesions, damage repair pathways. The error is in the new strand. How do prokaryotic systems deal with it: lesion: mismatch in the context of dna replication, figures out which one the new strand is (location of mistake) Uses markers to label the old strand. Ch3 (methyl a group) marks the parental strand. In e. coli, 7-megatc (methylation pattern in replication fork) Old template has a methyl group, new template has no methyl group: fixes the copy to match the template, figure 12-17. Binds to the methyl group near the lesion, which marks the template: figure 12-18. Muts binds mismatch: figure 12-17 part 2. Other activities come in after mapping of mismatch and determining between the 2 strands.
