9280 Lecture Notes - Lecture 3: University Of Cape Town, Amylase, Basal Metabolic Rate

A chromatography column is typically described by its number of theoretical plates, N, where N is defined by the equation N=16 (tr/w) 2 . The terms tr and σ are defined in Figure. Calculate N for each of the three peaks in the chromatogram in the picture and state whether N is constant.

The chromatogram below shows the separation of components in an over-the-counter pain reliever. 1: acetaminophen, tr 3.97 min, w1/2 0.12 min 2: caffeine, tr 6.15 min, w1/2 0.21 min 3: benzoic acid, tr 9.73 min, w1/2 0.35 min 4: aspirin, tr 14.8 min, w1/2 0.73 min Calculate the resolution R between acetaminophen and caffeine. Calculate the number of theoretical plates N for the peaks of acetaminophen and caffeine