BIO1022 Lecture Notes - Lecture 3: Reverse Transcriptase, Genomic Library, Dna Replication

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6 Jun 2018

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Lecture 3 (3/8): Molecular Biology

DNA: DNA replication

· Chromosomes = DNA and protein

Prokaryote & Eukaryotes

· Prokaryote and Eukaryotes differ in their replication

· This is due simplicity of their bacteria

· Origin of replication: where the copy of DNA starts

Replication fork

· Moves in both directions

· DNA grows in a 5’ à 3’ direction

· DNA polymerase 3 replicates DNA

Leading strand & Lagging strand

· Leading strand: Can add bases on the 3’ end going towards the replication fork

DNA ligase

· Sticks things together

Modification of an organism’s DNA

Lecture 4 (5/8): Molecular Analysis

Gene Technology/Genetic Engineering

Molecular cloning

· Genomic DNA: gDNA

Technique 1: Restriction Enzymes

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