BIOM20001 Lecture Notes - Cytochrome P450, Haematoxylin, Eosin
Document Summary
But due to increased exposure of smoke toxins, columnal cells changed to squamous epithelial cells (like those in gi tract lining) to better withstand the toxins. Factors affecting progression from reversible to irreversible injury. Injury factors: type, duration, severity low dose is less likely to trigger irreversible injury. Staining by dapi for transparent cells nuclei. Haematoxylin and eosin stain: most popular staining method used in histology, haematoxylin used as a basic dye so it is able to stain acidic structure (dna, Rna and rough er) in purpulish blue: eosin used as an acidic dye so it is able to stain basic structures (most proteins) in red or pink. Decreased atp (caused by ischemia to mitochondria) Damage to mitochondria (od mitochondrial dysfunction): reduced atp production: cytochrome protein c becomes pro-apoptotic (i. e. able to cause apoptosis) Increased production of ros (likely to make oxygen into ros instead of water when it is normally functioning)
