BIO SCI 98 Chapter Notes - Chapter 16: Disulfide, Pyruvic Acid, Deprotonation

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With glycine being in a more oxidized state, it now has a more positive charge and will want to react with other molecules because of its present state. An sds-page gel with 2-mercaptoethanol as the reducing agent was most likely used to obtain two bands. In an sds-page gel a polyacylamide gel is used to separate proteins and uses sodium dodecyl sulfate to give each protein fragment roughly the same charge per amino acid and to unfold the proteins. This allows the proteins to move through the gel according to size with an equal charge. The fragments would be placed at one end and would run down the gel towards the positive anode (since they have a slight negative charge) Smaller fragments would migrate further down the gel. The sds-page is important in that it gives us the ability to separate proteins, and protein subunits, by their mass. Glycolysis is the process responsible for creating g3p.

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