BIO130H1 Chapter Notes - Chapter 18: Fluorophore, Molecular Beacon, Hair Follicle
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BIO130H1 Full Course Notes
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In 1983, a new technique was discovered by kary mullis of cetus. Corporation: it has become widely used to amplify specific dna fragments without the need for bacterial cells. Pcr amplification is readily adapted to rna templates by first converting them to complementary dnas using reverse transcriptase. The mixture is then heated to about 95 c, which is hot enough to cause the dna molecules in the sample to separate into their two component strands. This cycle is repeated over and over again, each time doubling the amount of the specific region of dna that is flanked by the bound primers. Dna from minuscule samples, such as that in a single cell. This same procedure can be used to study dna fragments from. The activity of dna polymerase in pcr is also employed in dna sequencing. Testing for the presence of specific dna sequences. Sometimes, tissues are tested for the presence of viruses.
