BIOL130 Chapter 15: BIOL 130 Chapter 15- Protein Sorting and Intracellular Compartments

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coffeewildebeest729

14 Nov 2015

School

University of Waterloo

Department

Biology

Course

BIOL130

Professor

All

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Prokaryotic cells are enclosed by a single membrane. Eukaryotic cells have multiple organelles enclosed by a membrane. Nucleus: surrounded by a nuclear envelope (double membrane, communicates with cytosol through nuclear pores, outer membrane is continuous with er. Peroxisomes: contains enzymes used in oxidative reactions that break down lipids. Mitochondria/ chloroplast: surrounded by a double membrane, sites of oxidative phosphorylation or photosynthesis. Membranes of the membrane enclosed organelles originated from the plasma membrane. Any protein synthesized in the cytosol depends on the amino acid sequence, sorting signal, to direct it to where it is required. No sorting sequence= remains in the cytosol. Signal sequence is removed from a protein once it has been sorted. Inner nuclear membrane contains proteins that are binding sites for chromosomes. Nuclear pores form gates that molecules can enter or leave the nucleus. Large molecules must have a nuclear localization sequence that is recognized by nuclear.

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QUESTION 30

The function of centrosome is:

	1.	Microtubule initiation and organization in animal cells
	2.	Organization of thin filaments
	3.	Depolymerization of microtubules
	4.	Organization of intermediate filaments

2.5 points

QUESTION 31

The destinations of proteins synthesized on "free" ribomsomes include:

	1.	Endoplasmic reticulum
	2.	Nucleus
	3.	Mitochondria
	4.	Lysosomes

2.5 points

QUESTION 32

Microtubules can be assembled and disassembled quickly in response to the cellular changes is because:

	1.	Cells synthesize tubulin proteins very fast.
	2.	Cells can turn other cytoskeletal filaments into microtubules
	3.	Microtubules have an instable plus end and microtubules are probably organized by weak non-covalent interactions
	4.	The motor proteins of microtubules can organize the microtubules in response to the cellular changes

2.5 points

QUESTION 33

Both signal recognition particle and a translocon in rough endoplasmic reticulum membrane can recognize and bind to the signal sequence of a secretory protein, so they play the exact same role during protein co-translational translocation.

True

False

2.5 points

QUESTION 34

Rough endoplasmic reticulum is so named is because:

	1.	It has many proteins associated with its surface.
	2.	It has ribosomes associated with its cytosolic surface.
	3.	It is associated with nuclear membrane.
	4.	It has vesicles budding off its surface.

2.5 points

QUESTION 35

The membrane vesicles that transport materials between membrane compartments have a protein coat. The protein coat has similar functions in different vesicles. These functions include:

	1.	Help the enclosed material fold correctly.
	2.	Help degrade the protein in the vesicles
	3.	Maintain the internal pH of the vesicles
	4.	Function as mechanical device to form a vesicle and select the components to be carried by a vesicle.

2.5 points

QUESTION 36

Lysosomes are important for:

	1.	Receptor-mediated endocytosis.
	2.	Breaking down foreign particles brought into the cells by endosomes
	3.	Regulating the process of organelle turnover.
	4.	Protein trafficking between membrane compartments

2.5 points

QUESTION 37

Which of the following is correct about membrane biosynthesis?

	1.	Membranes are synthesized in Golgi Complex.
	2.	Membranes arise from preexisting endoplasmic reticulum membranes.
	3.	Membranes are synthesized on ribosomes.
	4.	Membranes of all organelles have the same composition of proteins and lipids, since they all originate from endoplasmic reticulum.

2.5 points

QUESTION 38

During skeletal muscle contractions, the shortening of the muscle fibers is because of:

		The shortening of the thin and thick myofilaments.
		The shortening of the cytoskeletal filaments.
		The kinesin proteins moving over microtubules.
		The actin and myosin filaments sliding over each other.

2.5 points

QUESTION 39

The assembly and disassembly of microtubules is regulated by rate of growth and shrinkage of the plus end.

True

False

2.5 points

QUESTION 40

The functions of Golgi complex include:

	1.	O-linked oligosaccharides and most of a cellâs complex polysaccharides are assembled in Golgi complex
	2.	Cis Golgi complex sorts proteins
	3.	Trans Golgi complex segregates proteins in different types of vesicles for dilevery.
	4.	N-linked oligosaccharides are further processed in Golgi complex

ochrecoyote721

Question 401 pts

Kinetic energy is the stored energy that can be used for motion.

True

False

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Question 411 pts

When an electron is transferred from one atom to another, and the two atoms are then electrically attracted to one another, a(n) ________________ bond is formed.

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Question 421 pts

Changing the number of ____________ of an atom would change the chemical properties of the atom.

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Question 431 pts

Forming molecules and breaking down molecules in biological organisms usually requires the use of _________ to help the reaction proceed faster.

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Question 441 pts

Organic molecules have a core composed of .

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Question 451 pts

The building blocks of carbohydrates are

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Question 461 pts

Water molecules crossing a membrane from high to low concentration is .

active transport

cell fate

facilitated diffusion

osmosis

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Question 471 pts

Choose the membrane molecule responsible for the passage of polar molecules and ions into and out of the cell.

phospholipids

transmembrane channel proteins

cell surface proteins

carbohydrate chains

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Question 481 pts

The simplest cells are .

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Question 491 pts

Which of the following membrane bound organelles are found inside bacterial cells:

nucleus

organelles are not found in prokaryotic cells

mitochondria

chloroplasts

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Question 501 pts

Which of the following is a specialized components of the cell, is associated with the rough endoplasmic reticulum, and is responsible for making proteins.

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Question 511 pts

Proteins are sorted, modified, and packaged by the _____________ and later transported to the outside of the cell.

ribosomes

golgi bodies (golgi apparatus)

nucleus

mitochondria

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Question 521 pts

Dehydration synthesis is a process of linking two smaller subunits together to form a polymer. Which of the following statements below is true of dehydration synthesis?

Oxygen is consumed.

A water molecule is removed from the molecules.

A water molecule is added to the molecules

Carbon dioxide is given off.

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Question 531 pts

Proteins are made up of _________ held together by peptide bonds.

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Question 541 pts

Which of the following gives an amino acid its properties?

NH2

R Group or functional group

COOH

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Question 551 pts

The final three-dimensional shape of a protein that includes the bonding of two or more polypeptide chains is call its _____ structure.

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Question 561 pts

How do DNA and RNA differ?

all statements are differences between DNA and RNA.

DNA is double stranded while RNA is single stranded.

They have different sugars.

Thymine is present in DNA but not in RNA.

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Question 571 pts

Which of the following is a component of a DNA nucleotide?

phosphate

a nitrogen containing base

All are components of a nucleotide.

5-carbon sugar

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Question 581 pts

Cell membranes are made up of several different types of molecules. Select the membrane molecule below that is made up of a polar region and two non-polar fatty acid tails.

transmembrane proteins

carbohydrate chains

phospholipids

cell surface proteins

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Question 591 pts

Two organelles which are believed to have once been free-living bacterial cells are ____________ and ______________.

chloroplasts and mitochondria

golgi apparatus and endoplasmic reticulum

ribosomes and nucleolus

peroxisomes and lysosomes

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Question 601 pts

The is an extensive system of internal membranes responsible for producing carbohydrates and lipids.

smooth endoplasmic reticulum

rough endoplasmic reticulum

nucleolus

mitochondria

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________30. If you are investigating the inner structure of a new cell and see that it has a significantly larger amount of RER than other cells. You would conclude the cell does a great deal of____?
A. ATP synthesis

B. protein synthesis
C. DNA synthesis
D. active transport
E. polysaccharide hydrolysis

________31. If a cell comes in contact with a toxic substance such as H2O2, it could survive as long as it has a ___ to digest it.
A. DNA
B. peroxisome

C. mitochondria
D. phospholipids
E. intermediate filaments

________32. Some cells have structures to help them withstand osmotic pressure. For example,
A. Plant cells due to their walls
B. Animal cells due to the sodium/potassium pumps

C. Bacterial cell due to their swimming

D. Viruses due to their protein coats

________33. Evolution occurs only as a cell ____.

A. They undergo mitosis
B. Change in their gene pool
C. They move from one habitat to another

D. They begin to eat different foods

________34. Ribosomes are produced in the

A. nucleoli.
B. chromatin.
C. nuclear envelopes.

D. Golgi complex. E. cisternae.

________35. Algae are often green, but there are red and brown species also. What accounts for this difference in color?
A. The chlorophyll of chloroplasts
B. The pigments in other plastids

C. The presence of a mitochondria
D. The accumulation of cellular wastes

________36. The chloroplast and other like prokaryotes have which of the following that helps with photosynthesis?
A. They live in water based environments
B. They have chloroplasts in their cytoplasm

C. Change color in the dark vs. the sun
D. They have photoreceptor proteins in their membranes or mesosomes.

________37. Located in the membrane, we see a set of proteins called receptors that only connect with or bind to specific substances. This is called __.
A. ligand specificity.
B. hormone selectivity.

C. cell tonicity.
D. water saturation. E. signal resolution.

________38. Of the following statements, we could say that ___ is false.
A. All organisms are composed of cells and cell products.
B. An organism's structure and all of its functions are due to the activities of cells.
C. The cellular organelles are the simplest structural and functional unit of life.
D. Cells come from preexisting cells.
E. Cells are made from nonliving components including the nucleus, cell membrane and cytoplasm.

________39. If it is really true that our cells are the smallest anatomical unit that meets all of the properties of life, then which of the following could imply otherwise?
A. vacuoles
B. centrioles

C. Golgi apparatus
D. mitochondria
E. endoplasmic reticulum

________40. Oxygen is an absolute need for animal and plant cells alike due to the ___.

A. RER
B. Mitochondria
C. Lysosomes

D. DNA

________41. In a plasma membrane, we expect to see the carb/protein structures to extend into the ___, but the secondary messengers to move within the __.
A. extracellular fluid; cytosol
B. cytosol; extracellular fluid

C. intracellular fluid; cytosol

D. cytosol; intracellular fluid

E. extracellular fluid; plasma

________42. Bacteria are found in all of the following habitats:

A. Caves
B. Oceans
C. Animals

D. Everywhere on Earth

________43. Two solutions are separated by a selectively permeable membrane. Solution A has a higher concentration of an impermeable solute compared to solution B. Which of the following do you expect would happen?
A. solute will move from solution A to solution B

B. solute will move from solution B to solution A

C. water will more from solution A to solution B

D. water will move from solution B to solution A E. no movement of solute or water will occur

________44. In the kidney, we notice that almost all of the cells involved in the production of urine have high concentrations of the Na+/K+ pump. Which typically works to push Na+ ___ its concentration gradient by using ___.
A. up; active transport

B. up; facilitated transport
C. up; cotransport
D. down; active transport
E. down; facilitated transport

________45. In the charts of a patient recently admitted to the hospital noted that he was taking a medication called lithium and high doses of calcium supplements. Given this, we would expect the patientâs liver to have a higher than normal amount of __ to purify out the lithium and calcium.

A. lysosomes.
B. centrosomes.
C. mitochondria.
D. peroxisomes.
E. rough endoplasmic reticulum.

________46. We have long observed that our neural cells in the brain cannot replicate or replace themselves due to the lack of ____.
A. produce ATP.
B. divide.

C. produce enzymes.
D. secrete material across their plasma membrane E. conduct electrical signals.

________47. An organismâs structure and function is ultimately due to the structure and function of itâs __.
A. Organs
B. Tissues

C. Cells

D. Nuclei

BIOL130 Chapter 15: BIOL 130 Chapter 15- Protein Sorting and Intracellular Compartments

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