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Biology And Biomedical Sciences BIOL 2960 Study Guide - Quiz Guide: Hydrolysis, Exonuclease, 16S Ribosomal Rna

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salmonyak403
18 Apr 2016
School
WUSTL
Department
Biology And Biomedical Sciences
Course
Biology And Biomedical Sciences BIOL 2960
Professor
Kunkel Barbara

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Document Summary

Transcriptional regulation of the lac operon, cycle-sequencing of 16s rrna gene pcr. Operon: group of genes that are transcribed in a single polycistronic mrna molecule. lacz: encodes beta-galactosidase which catalyzes the hydrolysis of lactose into glucose and galactose. Lac y: synthesizes permease that transports lactose into the cell. Lac a: codes for transacetylase that converts non-metabolizable beta-galactosides for excretion. The lac repressor binds to the operator when active. The repressor unbinds from the operator if it binds to lactose or mutates. Iptg (a derivative of lactose) binds to the repressor (is an inducer). Emb: ph sensitive dye that turns green when acidic and is red/purple when not. If cells use lactose in the presence of emb, the plate will be green (lactic acid). If they don"t, then the plate will be red/purple. X-gal: can be cleaved by beta-galactosidase but does not induce transcription. Iptg can induce transcription and be cleaved by beta-galactosidase.

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Related Questions

1.)A.)The lac operon is inducible by the presence of lactose. Loss-of-function mutations in the lacI gene, which encodes the lac repressor (pick all that apply):


A.)slow down the isomerization of lactose into allolactose
B.)decrease the amount of permease activity in the cell in the presence of lactose.
C.)prevent loss-of-function lacZ mutations from making the lac operon uninducible
D.)are recessive to wild type

B.)Mutations that eliminate the lacO operator site (pick all that apply):

A.)slow down the isomerization of lactose into allolactose
B.)decrease the amount of beta-galactosidase in the cell in the presence of lactose
C.)prevent lacS superrepressor mutations from making the lac operon uninducible
D.)are recessive to wild type

C.)Loss-of-function mutations in the lacZ gene, which encodes beta-galactosidase (pick all that apply):

A.)slow down the isomerization of lactose into allolactose
B.)decrease the amount of permease activity in the cell in the presence of lactose
C.)prevent lac repressor from binding the lacO operator site
D.)prevent IPTG from inducing the lac operon

D.)You are studying the Tory operon, which encodes the TorA and TorB genes, and is normally inducible by Torose. You find a mutation, m1, that causes the Tory operon to be uninducible. You examine the merodiploid (m1 TorA+ TorB-)/(+ ToryA- TorB+) and find that Tor A is uninducible and TorB is inducible. You conclude that:

A.)m1 acts in cis
B.)m1 acts in trans