BIOLOGY 190A Study Guide - Final Guide: Proinsulin, Insulin, Gene Expression
13 Jan 2016
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Insulin was first purified from animals why was this a problem: a major drawback to animal insulin is in the peak activity time. Short acting insulin derived from bovine serum will peak for only about 3 to 4 hours. Animal insulin is not exactly the same as human insulin. Just a difference of a couple of amino acids can induce an immune response. Clone cdna (insulin) into vector - primers allow for insertion. Insert plasmid into a bacterial cell and it will reproduce itself with the inserted gene when the bacteria divides. Human insulin is also less expensive than purified animal insulin. transcriptional or translational modification. The human cdna that is inserted into the recombinant plasmid would need to have been modified in a lab to remove introns before insertion. Have a solid understanding of how different major classes/types of receptors work (e. g. rtk, gpcr, notch: rtk - receptor tyrosine kinase.
