BIOL 1153 Study Guide - Quiz Guide: Alcaligenes Faecalis, Proteus Mirabilis, Electron Acceptor

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Quiz 4 covers and lab 8 (exercises 5-6, 5-7, 5-9, 5-11, 5-12, 5-18, 5-19, 5-24), lab 9 (exercises. 2-6 and 2-12) and lab 11 (exercise 6-1) Lab 8: differential (biochemical) tests: used for identification of bacteria by analysis of different biochemical properties; know what they test & how to interpret: Citrate test: green blue (positive: citrate as sole carbon source and ammonium phosphate as sole nitrogen source, simmons citrate agar is a defined medium, this tests for ability to use citrate as a sole carbon source. If the bacteria grows on citrate agar, the nitrogen source ammonium phosphate is converted to ammonium which turns the bromthymol blue ph indicator blue (basic: citrate test is part of the imvic tests for differentiating the enterobacteriaceae. Indole/methyl red/voges=proskauer/citrate: 2 agar slant tubes per group; stab inoculate with, proteus mirabilis, e. coli or bacillus, stab agar with loop, then run loop across the top of the slant.

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