DGS 3222 Study Guide - Quiz Guide: Mitosis, Down Syndrome, Trisomy

• School: UConn
• Department: Diagnostic Genetic Sciences
• Course: DGS 3222
• Professor: Martha Keagle

Document Summary

3-letter staining code: 1st letter type of banding, 2nd letter technique used for banding, 3rd letter specific stain used, exceptions. Solid staining: good for looking at frag sites. Gtg most commonly used for g-banding. Gtw sharper resolution on long chromosomes. Gtl produces better images on computer: dark regions at rich, light regions gc rich, slide-aging required. C-banding: stains constitutive heterochromatin around centromere (not the centromere) The rest of the chromosome is washed out: constitutive heterochromatin represents 20% of human genome, no banding down length of chromosomes, useful for studying. Marker chromosomes: c-band regions resist degradation by baoh used in c-banding. Nor: nucleolus organizing region located on satellite stalks of acrocentric chromosomes. Unique satelllites have been observed in 7 and y. R-banding: fluorescent method reverse of q-banding, used to visualize telomeres, non-fluorescent reverse of g-banding, used to study terminal ends of many chromosomes. Telomere banding: offshoot of r-banding, terminal ends of telomeres stained, fluorescent and nonfluorescent techniques exist.