MICROM 302 Study Guide - Final Guide: Lysogenic Cycle, Prophage, Macconkey Agar
MICROM 302 – LAB WRITTEN EXAM STUDY SHEET
Plates:
DIFFERENTIAL:
- Differential for hemolysis
oBlood agar
Highly nutritious
Beta hemolytic = complete clearing (YELLOW PLATE)
Strep throat
Alpha hemolytic (greenish)
- Differential for streptococcus
o5% SUCROSE + TSY
Form gum-drop like colonies
Polysaccharide capsules formed between streptococcus and sugar
DIFFERENTIAL AND SELECTIVE
- Selective for gram NEGATIVE RODS (whatever grows) / differential for lactose
fermenters (RED)
oEMB plate
selective for COLIFORMS
E. coli turns shiny glittery green
Enterobacter = dark purple circle with light encircling
oLauryl Lactose Broth
Detergent inhibits / gas production
oMacConkey Agar
Bile salts and dyes inhibit
RED = fermenter
- selective for halotolerant (high salt concentration staphylococcus) / differential for
mannitol fermenters (YELLOW)
oMannitol Salt Plate
Stays pink = non mannitol fermenter
OTHER
- Not differential or selective
oTSY Agar Plate
oMueller-Hinton Plate (more defined media / more reliable)
- Other plates
oTryptone Base Plates
Provide extra moisture and nutrients
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Document Summary
Microm 302 lab written exam study sheet. Beta hemolytic = complete clearing (yellow plate) Differential for streptococcus: 5% sucrose + tsy. Selective for gram negative rods (whatever grows) / differential for lactose fermenters (red: emb plate selective for coliforms, coli turns shiny glittery green. Enterobacter = dark purple circle with light encircling: lauryl lactose broth. Detergent inhibits / gas production: macconkey agar. Red = fermenter selective for halotolerant (high salt concentration staphylococcus) / differential for mannitol fermenters (yellow: mannitol salt plate. Not differential or selective: tsy agar plate, mueller-hinton plate (more defined media / more reliable) To titer viruses / phages (plaques: czapek"s agar. To see antibiotic production streptomyces!: sea water complete agar. Allows growth / provide nutrients for marine life. Lytic/virulent phages: always lyse their host cell: form clear plaques in tryptone soft agar. Temperate/lysogenic phages: sometimes lyse their host cell, other times become integrated into host cell: repressor protein: repress lytic genes makes it stay in lysogenic cycle.
