MICROM 302 Study Guide - Quiz Guide: Pilot Light, Bunsen Burner, Biological Hazard
MICROM 302 – QUIZ 1 NOTES
Safety in the Lab:
Where is the closest fire extinguisher?
oBy the door to the hallway with the lab coats
Where is the closest eyewash?
o
Where is the closest safety shower?
o
Aside from the laboratory regulations listed in the manual, where else is the laboratory safety
information kept?
oCANVAS
What are two reasons why proper aseptic technique should be used at all times when
handling microorganisms?
oSo that your culture won't become contaminated with organisms from the surrounding
environment
You won't be able to identify what you actually want
oAlso important to not contaminate yourself or your surroundings with the culture
Protecting you and the environment from possibly harmful bacteria
Disposal of Materials (contaminated and not contaminated):
What must be done to all contaminated materials before they are discarded into the regular
garbage?
oEither disinfected
Why are plastic Petri dishes discarded into an autoclave bag, while glass tubes with plastic
caps are discarded directly into the bin?
oGlass tubes are washed and reused
oPetri dishes are discarded into the orange biohazard bag because they are contaminated
Where do you dispose of plastic pipettes and swabs?
oIf they have been contaminated, you throw them away in the autoclave bag
Where do you dispose of the wrappers from pipettes and swabs?
oYou can just throw these away if they haven't been contaminated by anything. The
wrappers themselves are sterile.
Where do you dispose of the disinfectant-soaked paper towels that you use to swab your
desk?
oIn the orange biohazard bags
Where do you dispose of the disinfectant-soaked paper towels that you use to clean up a
spill?
o
With respect to using a Bunsen burner:
find more resources at oneclass.com
find more resources at oneclass.com
Document Summary
Where is the closest fire extinguisher? o. By the door to the hallway with the lab coats. Where is the closest safety shower? o. Aside from the laboratory regulations listed in the manual, where else is the laboratory safety information kept? o. What are two reasons why proper aseptic technique should be used at all times when handling microorganisms? o. So that your culture won"t become contaminated with organisms from the surrounding environment. You won"t be able to identify what you actually want: also important to not contaminate yourself or your surroundings with the culture. Protecting you and the environment from possibly harmful bacteria. What must be done to all contaminated materials before they are discarded into the regular garbage? o. Why are plastic petri dishes discarded into an autoclave bag, while glass tubes with plastic caps are discarded directly into the bin: glass tubes are washed and reused o.
