01:447:384 Study Guide - Final Guide: Restriction Enzyme, Agarose Gel Electrophoresis, Restriction Digest
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Problems involving restriction enzymes: a piece of linear dna contains the palindrome ctgcag. This palindrome is randomly repeated 3 times along a piece of linear dna. A restriction digest is made that includes this piece of dna and the restriction enzyme that cuts the ctgcag palindrome. i. How many fragments will be generated once dna is fully digested?________________ ii. Are the fragments of the same size or various sizes (choose one). ___________________________________________________________________________________________: the image below is showing a piece of linear dna that is being cut by two different restriction enzymes a and b. The length of dna is a total of 456 bp long. Enzyme a cuts the dna at position 90 bp and enzyme b cuts the dna at 400 bp. i. A solution containing the above restriction digest is place in a well of the agarose gel at the right. Draw the migration pattern formed by the pieces. Also label the electrodes with their appropriate charge. iii.
