GENE 540 Study Guide - Midterm Guide: Cyclin-Dependent Kinase 1, Protein Kinase, Interphase
Document Summary
They all stopped at g2 for cdc2- mutants all cells are the same size at the non- permissive temperature arrested at g2. This means that cdc2- is required for the cell to go to the m phase. Cdc2 is a serine/threonine protein kinase that is required for cells to go into m-phase. There is a gene in humans that help cells go from g2 to m this means that eukaryotic cell cycle regulatory machinery is high conserved! The complex processes of mitosis are initiated by a regulator that is functionally conserved from yeast to man. M-phase cells: microscopic observation - cells in m phase are easily distinguished from cells in interphase, most mitotic cells change shape, round up, and detach from the substrate, chromosomes are condensed (visible by light microscopy). Staining for microtubules reveals a spindle: cultured cells its harder to distinguish between phases; but you can find out if its in mitosis compared to interphase.
