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BIO_SC 2200 Study Guide - Midterm Guide: Sister Chromatid Exchange, Dna Polymerase Iii Holoenzyme, Sister Chromatids

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crimsonfox254
27 Apr 2016
School
Mizzou
Department
Biological Sciences
Course
BIO_SC 2200
Professor
Dr.David

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Related Questions

Which of the following are required for DNA replication by DNA polymerase?

A. Template strand
B. Short, complementary primer strand
C. 5'-dXTPs
D. 3'-dXTPs
E. Answers (A) and (C)
F. Answers (A) and (D)
G. Answers (A), (B), and (C)

H. Answers (A), (B), and (D)

Which of the following are reasons why DNA replication must be discontinuous?

A. The complementary strands have opposite polarity
B. Only 5'-dXTPs are available in cells
C. DNA polymerase only adds 5-'dXTPs to 3'-OH ends
D. All the above
E. Answers (A) and (C)
F. Answers (B) and (C)

G. Answers (A) and (B)

Which of the following are roles of RNA polymerases in DNA replication?

A. RNA polymerase (primase) initiates replication at the origin
B. RNA polymerase synthesizes the primers for Okazaki fragments
C. Both (A) and (B)

D. Neither (A), nor (B)

Which of the following statements describes the activity of DNA polymerase?

A. DNA polymerase adds 3’-dXTPs to the 5’-OH terminus of the primer
B. DNA polymerase adds 5'-dXTPs to the 3’-OH terminus of the primer
C. DNA polymerase adds 5’-dXTPs to the 3’-phosphate terminus of the primer
D. DNA polymerase adds 3’-dXTPs to the 5’-phosphate terminus of the primer

1) What is the difference between the leading strand and the lagging strand in DNA replication? Place the following steps of DNA replication in right chronological order.

1. Primase adds an ribonucleotide primer
2. Initiator proteins bind the origin of replication and helicase breaks hydrogen bonds between complementary bases of double stranded template DNA
3. DNA polymerase III extends by complementary base pairing deoxyribonucleotides to the template strands
4. Ligase phosphodiester bonds the nucleotides to connect okazaki fragments nad connect fragments at the origin and where the "crotches or forks" collide
5. DNA polymerase I removes RNA primer and replaces with Deoxyribonucleotides


2) Given the template DNA and primer sequences below, what bases will be added to the primer as DNA replication proceeds? (The bases should appear in the order that they will be added)

5'-GTCGTCTCCCTTTTTAAAAACCCCCCC-3'
5'-UUUUUAAAAAGGG-3'


3) Given the DNA sequence below, what could be a primer sequence used in its replication?
5'-CGTACGGCCCCAAAATTGCTACTCTGCTG-3'

Order the following steps of the DNA replication process fromfirst to last. (From 1 to 8)

DNA ligase joins Okazaki fragmments together

Elongation of leading strands occurs continuosly and elongationof lagging strands occurs via generation of Okazaki fragments. Bothare synthesized in 5' to 3' direction.

DNA polymerase I removes the RNA primers and replaces them withDNA nucleotides

DNA polymerase III starts adding DNA nucleotides to the 3' endof the existing RNA chain.

Helicase untwists the double helix at the replication fork

Single-stranded binding proteins bind the unpaired DNA strands,keeping them from re-pairing.

Topoisomerase helps releive the strain caused by DNA untwisting.It breaks, swivels and rejoins DNA strands.

Primase starts a complementary RNA chain using parental DNAstrand as a template