BIOL 150 Study Guide - Midterm Guide: Aids, Pipette, Pyruvic Acid

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30 Oct 2014

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Scientific method: make an observation, form a hypotheses. Idea generated to explain a given observation or phenomenon: test the hypotheses, state the results of the testing, including your interpretations about what the results mean, retest old hypothesis or form a new one. Independent variable: anything intentionally changed by the scientists, put on the x-axis. Dependent variable: changes in response to the independent variable, put on the y-axis. Levels of treatment: the different values of the independent variable. Remember, the more objects in the study the better the results. Aids (acquired immunodeficiency syndrome: a weakened immune system (hiv, become seriously ill within 5-10 years after infection, passed through non-casual human contact, hiv is a retrovirus. Mutate very rapidly: can take treatments like azt and a pill cocktail to delay effects. Beakers and flasks: used to measure larger volumes of solution, not accurate for precise measurements of ml (graduated cylinders are more accurate for measurements of large volumes)

Related Questions

Read the following passage focusing your attention on the scientific procedures and scientific abilities illustrated in it. Think about what types of experiments (observation, testing or application) are described. Identify the hypothesis, predictions, outcomes of experiments and assumptions that appear in the passage. The standard explanation of how humans originally acquired AIDS is that HIV (human immunodeficiency virus) originally was transmitted to humans from monkeys or apes carrying SIV, simian immunodeficiency virus. How could the transmission have occurred? Several similar hypotheses involve an African hunter coming in contact with monkey blood. For example this could have occurred when a hunter butchering a monkey, got some of its blood in a cut, or when a human ate undercooked monkey meat, or when a monkey bit a human. Depending on one's assessment of the genetic diversity of HIV over time, this transmission might have occurred only a few decades ago or, alternatively, hundreds or thousands of years ago. If the latter is correct then AIDS could have been restricted to remote African villages until recent decades when trade and international travel led to the current pandemic. There are however other alternative explanations for the spread of AIDS. One of them is that AIDS arose from contaminated polio vaccines used in Africa from 1957-59. Why is this a plausible explanation? Polio vaccines are cultured on apesâ kidneys, so contamination of a batch of vaccine by an SIV is a possibility. There is a precedent: in the 1950s and early 1960s millions of doses of polio vaccine were contaminated by another simian virus, SV-40. The vaccine alleged to be the origin of AIDS was given to hundreds of thousands of people in central and west Africa from 1957-1959. These locations later had some of the world's highest levels of HIV infection. The timing is also right, since the earliest known cases of AIDS date from the 1960s; the earliest HIV-positive blood sample was collected in Kinshasa in 1959. At the time, there was no way of testing for the presence of SIVs, which were only discovered in the 1980s. Another factor adds plausibility to the theory: the vaccine was given to many infants less than a month old, in an extra high dose. Since the immune systems of infants are not fully developed, this is an excellent way to help a virus jump the species barrier. Some scientists argue that the above explanation is not necessarily correct but that it is worth taking seriously. There are a number of ways to test it. One is to analyze stocks of polio vaccine; if the explanation is correct, then it is probable that some of the vaccines will carry SIV. Another is to scrutinize records of early polio vaccination campaigns in Africa. If it is true that the immunodeficiency virus passed to humans by way of those vaccines, the areas where the campaigns took place would be the same as those where the initial sprouts of the disease occurred and where the larger number of cases occurred during the early stages of the pandemic. Another is to analyze blood samples in Africa from the 1950s and earlier (pre polio vaccination): if the immunodeficiency virus was transmitted via the polio vaccine, HIV should not be found. Adapted from âPolitical refutation of a scientific theory: the case of polio vaccines and the origin of AIDSâ. Brian Martin, published in Health Care Analysis, Vol. 6, 1998, pp. 175-179. Now reread the passage and answer the following questions.

5. For each experiment, write an outcome that would disprove the hypothesis of AIDS passing from monkeys to humans through polio vaccines.

6. What outcomes of the experiments would fail to disprove the hypothesis of AIDS passing from monkeys to humans through polio vaccines?explain.

7.What outcomes would prove the hypothesis? Hint: This is not the same as question 6. Think about what would you need to do to 100% prove a hypothesis.

8. For what reasons do scientists need to conduct testing experiments?

ochrejackal75

Question1.1. Accidental alterationsduring DNA replication that give rise to variations in organismsare __________. (Points : 1)

adaptive traits
mutations
symbioses
random distributions

Question2.2. The largest reservoir ofcarbon in the carbon cycle is comprised of _________. (Points :1)

freshwater systems andoceans
plants and animals
sedimentary rock andfossil fuels
atmosphere

Question3.3. The principle of utility________. (Points : 1)

holds that any naturalresource should be available for use in manufacturing
holds that something isright when it produces the greatest benefit for the greatest numberof people
is not an ethicalstandard
cannot be used toestimate the value of natural resources

Question4.4. The two processes thatdetermine current biodiversity on a global scale are __________.(Points : 1)

allopatric speciation andimmigration
mutation and habitatselection
mutation and globalclimate change
extinction andspeciation

Question5.5. The conservation ethicholds that we should ________. (Points : 1)

exploit natural resourcesfor economic gain regardless of sustainability
be prudent and efficientin the use of natural resources
consider the environmentthe ultimate servant of mankind
maintain the environmentin a pristine, unaltered state

Question6.6. The preservation ethicholds that we should ________. (Points : 1)

be responsible for wisemanagement while using natural resources
protect areas againstdevelopment while allowing public access
harvest naturalresources
maintain the naturalenvironment in a pristine, unaltered state

Question7.7. Trophic levels and foodchains diagram __________. (Points : 1)

logistic curves
energy flow in anecosystem
population density
natural resourcefluctuations

Question8.8. A hypothesis is ________.(Points : 1)

a prediction
an educated guess thatexplains a phenomenon or answers a question
an instrument that isused to examine environmental conditions
the design of anexperiment that can be used for the process of science

Question9.9. A biocentric view of theworld emphasizes ________. (Points : 1)

the role of corporationsin environmental law
the importance ofconsidering environmental impacts on all living things
the importance ofeconomics in environmental decisions
the dual-natured view ofhumans and animals as environmental controls

Question10.10. Malthus was responsiblefor ________. (Points : 1)

the book The PopulationBomb that described the disastrous effects of human populationgrowth
the idea that theincrease in human population would lead to famine and war
the concept that humanpopulation growth would lead to greater industry and prosperitythrough education
instituting fertilizeruse for agriculture

Question11.11. Ecology is ________.(Points : 1)

the study of how to solveenvironmental problems
the study of organismsand their interactions with each other and the environment
a subfield ofenvironmentalism
the study of the E. colifamily of bacteria

Question12.12. The scientific processis based on ________. (Points : 1)

observations thatresearchers keep in a scientific journal
testing hypotheses builton observation, and revising them based on results
intuitive understandingof natural laws and processes
the ancient writings ofHeraclitus

Question13.13. Which of the followingis an abiotic factor? (Points : 1)

Amphibians
Wood
Mammals
Water

Question14.14. In environmentalscience, "carrying capacity" means the ___________. (Points :1)

maximum population sizethat a given environment can sustain
average number of viableoffspring produced within a population
potential number ofspecies in a given area
limitation on the numberof species in an ecosystem

Question15.15. The Cuyahoga River madepeople aware of environmental problems because __________. (Points: 1)

people in manycommunities became sick when using water from the river
the drinking water inelementary schools near the river became contaminated
the river caught onfire
the river stoppedsupporting any plant or animal life

Question16.16. The conversion processwhere nitrogen gas combines with hydrogen to form ammonium ions,which is accomplished through the intense energy of lightening orwhen air in the top layer of soil comes into contact with certainbacteria, is known as ________. (Points : 1)

the carbon cycle
nitrogen fixation
ionic conversation
bacterial ionization

Question17.17. The fresh water wedepend on for survival accounts for approximately __________ of allwater on Earth. (Points : 1)

two-thirds
one-third
10%
3%

Question18.18. A paradigm is ________.(Points : 1)

a group of severalhypotheses that can be tested together
a dominant view inscience
the same as ahypothesis
synonymous with thescientific method

Question19.19. The degree of disorderin a substance, system, or process is called ________. (Points :1)

chaos
entropy
energy
electricity

Question20.20. A population is definedas ___________. (Points : 1)

a group of individuals ofmultiple, interacting species that live in a particular area
a group of similarspecies that inhabit various ecosystems
a group of individuals ofa single species inhabiting a particular area
(crude birth rate +immigration rate) - (crude death rate - emigration rate)

periwinklecoyote319

Chose the correct answers on one through 15(plese help)

1.A research group hypothesizes that successful infection by HIVdepends upon the expression of FSH3, a human gene. They overexpressthis gene in T cells (Which HIV normally infects) so that FSH3protein levels are very high in these cells. In other T-cells, theymutate FSH3 so that none of its encoded protein is made. They thenmeasure HIV infection rate in the control, overexpression, andmutant T cells. The three cultures are otherwise grown in the sameway.

What is the independent variable(s) in this experiment? (Selectall correct answers)

		FSH3 protein levels
		The medium in which cells are grown
		The temperature at which cells are grown
		HIV resistance of cells

Question 2

Which arises from an error in a measurement or observation?

		Technical variability
		Biological variability
		Standard error
		Standard deviation

Question 3

Use the following description of an experiment to answer thenext question.

A research group hypothesizes that successful infection by HIVdepends upon the expression of FSH3, a human gene. They overexpressthis gene in T cells (Which HIV normally infects) so that FSH3protein levels are very high in these cells. In other T-cells, theymutate FSH3 so that none of its encoded protein is made. They thenmeasure HIV infection rate in the control, overexpression, andmutant T cells. The three cultures are otherwise grown in the sameway.

What is the control group(s) in this experiment? (Select allcorrect answers)

		The unaltered T cells
		The medium in which cells are grown
		The T cells in which FSH3 is mutated
		The T cells overexpressing FSH3

Question 4

How many of the following statements are true?

I. All papers have an abstract, methods, results, and discussionsection

II. The methods always follows the introduction

		0
		2
		1

Question 5

If scientific convention states that when a P value of less than0.05 is considered significant (in other words, the two groupsbeing measured are actually different), this means that: (Selectall correct answers)

		If you were to repeat the experiment 100 times, there would notbe a significant difference between the groups in 5 of theexperiments
		95% of all conclusions that state two groups are not differentbased on a 0.5% p-value threshold are false negatives
		The groups are actually not different from each other 95% of thetime
		5% of all conclusions that state two groups are different basedon a 0.5% p-value threshold are false positives

What information is typically found in the introduction sectionof a paper? (Select all that apply)

		The dependent and independent variables within the study
		Which areas of the field are not well understood and whatquestion the study addresses
		How the main question of the paper is related to other researchin the field.
		How previous work forms the basis for the current study
		The purpose of the study
		Details of the approaches used
		A summary of relevant related studies

Question 7

How can you judge for yourself whether the conclusions reachedin a paper are valid? (Select all that apply)

		Read papers of other authors studying the same question or areaof inquiry
		Determine if each hypothesis is supported by different types ofevidence/approaches.
		Consider closely whether the data actually both addresses andsupports the authorâs hypothesis.
		Examine the amount of data in the paper, and how long the paperis.
		Whether the authors find a mechanism, or exactly how on variableaffects another.
		Interpret the data for yourself without looking the discussion,then compare your interpretations to that of the authorâs.

Question 8

Where can you usually find the institutional affiliations (wherethey work) of the authors?

		Within the discussion
		In the acknowledgements section
		Below the authors list
		This is not usually included in papers

Question 9

Other than publishing primary research articles, what is themain way scientists disseminate their results?

		Lab meetings
		Personal correspondence
		Conference seminars
		Public lectures

Question 10

Use the following description of an experiment to answer thenext question.

What is the experimental group(s) in this experiment? (Selectall correct answers)

		Each group is its own control
		The T cells overexpressing FSH3
		The T cells in which FSH3 is mutated
		The unaltered T cells

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BIOL 150 Study Guide - Midterm Guide: Aids, Pipette, Pyruvic Acid

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